DNA sequencing is the process of determining the precise sequence of nucleotides in a DNA molecule. This technology allows scientists to read and decipher the genetic code of an organism, providing valuable information about its genetic makeup, including genes, mutations, and variations. DNA sequencing has revolutionized fields such as genetics, genomics, and molecular biology, enabling researchers to study the molecular basis of diseases, evolutionary relationships, and genetic diversity. There are different methods for DNA sequencing, each with its own advantages and limitations, such as Sanger sequencing, next-generation sequencing (NGS), and third-generation sequencing technologies. DNA sequencing has had a profound impact on various areas of science and medicine, leading to breakthroughs in personalized medicine, agriculture, forensics, and environmental research.